Dogodki
> pretekli dogodki > Comprehensive transfectio...
Comprehensive transfection solutions for your research
Amaxa in Mediline
Ljubljana
9.12.2004
predstavitev
Successful gene transfer and gene silencing in primary cells and cell lines using Amaxa's Nucleofector™ technology.
The availability of genetically altered cells is an essential prerequisite for many scientific and therapeutic applications including functional genomics, drug development and gene therapy. Unfortunately, the efficient gene transfer into primary cells is still problematic. In contrast to transfections of most cell lines, which can be successfully performed using a variety of methods, the introduction of foreign DNA into primary cells requires a careful selection of gene transfer techniques. Whereas viral strategies are time consuming and involve safety risks, non-viral methods proved to be inefficient for most primary cell types. The Nucleofector™ technology is a novel gene transfer technique designed for primary cells and hard-to-transfect cell lines. This non-viral gene transfer method is based on a cell type specific combination of electrical parameters and solutions. Data and applications showing the efficient transfer of DNA expression vectors and siRNA oligonucleotides into a variety of primary cell types from different species utilizing the Nucleofector™ technology will be outlined.
For more information please visit www.amaxa.com.
Program predavanja
- Introduction in the nucleofector technology
- Cell line nucleofector
- Primary cells
- siRNA application
- Other applications - stable transfection, antibiotics against contamination
- Questions and discussion
Predavatelj
dr. Bernd Neufeld, Amaxa
Kdaj
v četrtek, dne 9.12.2004 ob 10:00 uri
Kje
Hotel Domina Grand Media Ljubljana*
konferenčna dvorana Middle Hall - Janus
Dunajska 154, Ljubljana
*Novi hotel poleg Smeltove stavbe / poleg WTC-ja. Parkiranje je možno v Smeltovi garažni hiši.
Registracija udeležencev
od 9:30 do 10:00 ure
Predvideno trajanje predavanja
od 1,0 - 1,5 ure
Rok za prijavo
do 30.11.2004
Kotizacija
Kotizacije ni.
Jezik
Predavanje bo potekalo v anglešcini.